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. 2013 Oct 23;64(18):5429–5441. doi: 10.1093/jxb/ert312

Fig. 7.

Fig. 7.

OsERdj3A localizes at PB-IIs in transgenic seeds expressing hIL-7. (A) Left panels show localization of ERdj3A (a) and OsTIP3 (d); middle panels (b and e) show localization of PB-Is (red); and right panels (c and f) show the merged images of left and middle panels (bar=10 µm). (B) (g, h, and i) Double staining images of hIL-7 seeds immunodecorated with anti-ERdj3A (g in green) and anti-glutelin B (GluB; h in red). (i) A merged image of g and h. Bar=5 µm. (C) OsERdj3A localizes to the vacuole in protoplasts under ER stress conditions. An ER-localized mCherry (SP-mCherry-HDEL) fusion construct was transiently co-transfected with J-protein–GFP in rice protoplasts. After 18h of incubation, the protoplasts were treated with 5 µg ml–1 tunicamycin for an additional 6h. Cells were observed and photographed with a confocal laser-scanning (GFP and mCherry) and a phase contrast (PC) microscope. Bar=10 µm.