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. 2013 Oct 11;64(18):5569–5586. doi: 10.1093/jxb/ert328

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Representative Pro-Q DPS-stained 2D-IEF/SDS-PAGE gels of the total soluble proteins in the control (–PEG) and PEG-treated root tips of common bean genotype VAX 1 (A) and three magnified spots with PEG treatment-enhanced protein abundance (B). Plants were pre-cultured in a simplified nutrient solution containing 5mM CaCl₂, 1mM KCl, and 8 µM H₃BO₃ for 48h for acclimation and pH adaptation, and then treated without or with PEG (150g l^–1) in the simplified nutrient solution (pH 4.5) for 24h. Proteins were extracted from the root tips, separated by 2D-IEF/SDS-PAGE, and stained by Pro-Q DPS. Treatment-affected spots were marked by arrows and numbered consecutively. Three independent biological replications of each treatment were analysed using the Image Master^TM 2D PLATINUM Software 6.0. (This figure is available in colour at JXB online.)