Fig. 5.

Cellular immunolocalization of dehydrin in the root tips of common bean. Plants were pre-cultured in a simplified nutrient solution containing 5mM CaCl₂, 1mM KCl, and 8 µM H₃BO₃ for 48h for acclimation and pH adaptation, and then treated without or with PEG (150g l^–1 PEG 6000) in the simplified nutrient solution (pH 4.5) for 24h. Transverse root sections were treated with the anti-dehydrin polyclonal antibody and secondary anti-rabbit IgG–FITC. The images were captured with a confocal laser-scanning microscope (see Materials and methods). (A–F, M, O) Fluorescence of dehydrin; (G–L, N, P) merged image of dehydrin.(A, G, D, J) root apical 0–3mm segments; (B, H, E, K) root apical 3–6mm segments; (C, I, F, L) root apical 6–10mm segments; (M, N) close-up of immunostaining of dehydrin in root apical 3–6mm segments; (O, P) close-up of immunostaining of dehydrin in root apical 3–6mm segments after plasmolysing the cells with 0.8M manitol. (A–C, G–I) No PEG treatment (–PEG); (D–F, J–P) PEG treatment (+PEG). Bars, 50 µm (A–L); 20 µM (M–P).