Fig. 6.

Fluorescent western blotting of dehydrin protein in the root tips of common bean. Plants were pre-cultured in a simplified nutrient solution containing 5mM CaCl₂, 1mM KCl, and 8 µM H₃BO₃ for 48h for acclimation and pH adaptation, and then treated without or with PEG (150g l^–1 PEG 6000) in the simplified nutrient solution (pH 4.5) for 24h. The proteins from root tips treated without and with PEG were fractionally extracted with protein extraction buffer without and with 1% (w/v) SDS (see Materials and methods). Lanes: A, fraction I of –PEG; B, fraction I of +PEG; C, fraction II of –PEG; D, fraction II of +PEG; E, fraction III (+1% SDS) of –PEG; F, fraction III (+1% SDS) of +PEG.