Table 1.
List of the 22 proteins with significantly different abundance in the root tips of common bean genotype VAX 1 in response to PEGPlants were pre-cultured in a simplified nutrient solution containing 5mM CaCl2, 1mM KCl, and 8 µM H3BO3 for 48h for acclimation and pH adaptation, and then treated without or with PEG (150g l–1) in the simplified nutrient solution (pH 4.5). These 22 protein spots are shown in the CBB-stained 2D-IEF/SDS-PAGE gels in Fig. 2 and Supplementary Fig. S1. Spot no. relates to the corresponding spot number of Figs 2 and S1. FC, fold change (the relative protein spot volume of +PEG treatment to the relative protein spot volume of –PEG treatment). The proteins were identified by nano LC-MS/MS and BLASTed in the NCBI database. The protein functions were categorized based on the UniProt database and KEGG pathway. NI, not identified; MW, molecular weight. See Supplementary Table S1 at JXB online for further details.
| Spot No. | Identity | MW (Da) | GenBank acc. no. | FC |
|---|---|---|---|---|
| Carbohydrate metabolism | ||||
| 4 | Acetoacetyl-CoA thiolase (Medicago sativa) | 41 659.8 | ACX47470 | 0.410 |
| 5 | Fructokinase (Arachis hypogaea) | 20 047.7 | ACF74294 | 0.411 |
| 7 | myo-Inositol 1-phosphate synthase (Phaseolus vulgaris) | 56 431.4 | CAH68559 | 0.441 |
| 9 | Phosphoglycerate mutase (Solanum tuberosum) | 60 271.9 | AAD24857 | 0.484 |
| 10 | Fructokinase-like protein (Cicer arietinum) | 26 092.1 | CAD31714 | 0.544 |
| 16 | Alcohol dehydrogenase Adh-1 (Glycine max) | 34 407.4 | AAC62469 | 2.425 |
| 17 | Enolase (Glycine max) | 47 701.9 | AAS18240 | 2.341 |
| 18 | Enolase (Glycine max) | 47 701.9 | AAS18240 | 1.986 |
| 22 | NADPH-specific isocitrate dehydrogenase (Glycine max) | 49 124.2 | AAA33978 | 1.500 |
| Amino acid metabolism | ||||
| 1 | S-Adenosylmethionine synthetase (Phaseolus lunatus) | 43 041.9 | BAB83761 | 0.190 |
| 2 | Methionine adenosyltransferase (Pisum sativum) | 40 958.3 | CAA57581 | 0.245 |
| 3 | S-Adenosylmethionine synthetase (Phaseolus lunatus) | 43 041.9 | BAB83761 | 0.307 |
| 6 | d-3-phosphoglycerate dehydrogenase. putative (Ricinus communis) | 63 086.7 | EEF43612 | 0.440 |
| 20 | S-Adenosylmethionine synthetase (Phaseolus lunatus) | 43 041.9 | BAB83761 | 1.514 |
| 21 | Methionine synthase (Glycine max) | 84 266.4 | AAQ08403 | 1.500 |
| Other metabolisms | ||||
| 12 | 1-Deoxyxylulose 5-phosphate synthase (Chrysanthemum x morifolium) | 71 701.7 | BAE79547 | 0.615 |
| 13 | Inorganic pyrophosphatase, putative (Ricinus communis) | 33 895.5 | EEF44062 | 0.619 |
| Stress response/defence | ||||
| 8 | Chloroplast thylakoid-bound ascorbate peroxidase (Vigna unguiculata) | 39 791.4 | AAS55852 | 0.596 |
| Protein processing | ||||
| 11 | Tubulin A (Glycine max) | 49 552.7 | AAX86047 | 0.566 |
| 19 | 26S proteasome regulatory particle triple-A ATPase subunit1 (Oryza sativa Japonica group) | 46 667.0 | BAB17624 | 1.910 |
| Transcription | ||||
| 14 | Pre-mRNA-splicing factor SLU7-A (Arabidopsis lyrata subsp. Lyrata) | 61 961.8 | EFH64697 | 2.794 |
| Unknown | ||||
| 15 | NI | – | – | 2.625 |