Table 3.
List of 13 apoplastic proteins with significantly different abundance in the root tips of common bean genotype VAX 1 in response to PEGPlants were pre-cultured in a simplified nutrient solution containing 5mM CaCl2, 1mM KCl, and 8 µM H3BO3 for 48h for acclimation and pH adaptation, and then treated without or with PEG (150g l–1) in the simplified nutrient solution (pH 4.5). These 13 protein spots are shown in the CBB-stained 2D-IEF/SDS–PAGE gels of Figs 3 and Supplementary Fig. S3. Spot No. relate to the corresponding spot number in Fig. 3 and in supplemental Fig. S3. FC: fold change (the relative protein spot volume of +PEG treatment to the relative protein spot volume of -PEG treatment). The proteins were identified by nano LC-MS/MS and BLASTed in NCBI database. NI, not identified; MW, molecular weight. See Supplementary Table S1 at JXB online for further details.
| Spot no. | Identity | MW (Da) | GenBank acc. no. | FC |
|---|---|---|---|---|
| 1 | NI | – | – | 0.486 |
| 2 | NI | – | – | 0.607 |
| 3 | Fructokinase (Arachis hypogaea) | 20 047.7 | ACF74294 | 0.636 |
| 4 | Pathogenesis-related protein 1 (PvPR1) (Phaseolus vulgaris) | 16 511.4 | CAA43637 | 0.635 |
| 5 | NI | – | – | 0.616 |
| 6 | Beta xylosidase (Fragaria×ananassa) | 83 468.2 | AAS17751 | 1.768 |
| 7 | NI | – | – | 2.067 |
| 8 | NI | – | – | 2.136 |
| 9 | Pectinacetylesterase precursor (Vigna radiata var. Radiata) | 43 804.9 | CAA67728 | 1.730 |
| 10 | Serine hydroxymethyltransferase, putative (Ricinus communis) | 51 889.9 | XP_002522806 | 1.935 |
| 11 | Serine carboxypeptidase, putative (Ricinus communis) | 50 034.7 | XP_002521402 | 1.709 |
| 12 | Serine hydroxymethyltransferase (Gossypium hirsutum) | 51 889.9 | ACJ11726 | 1.771 |
| 13 | Fructose-1.6-bisphosphate aldolase (Pisum sativum) | 38 473.4 | CAA61947 | 1.692 |