Table 4.
List of 10 phosphoproteins with significantly different abundance in the root tips of common bean genotype VAX 1 in response to PEGPlants were pre-cultured in a simplified nutrient solution containing 5mM CaCl2, 1mM KCl, and 8 µM H3BO3 for 48h for acclimation and pH adaptation, then treated without or with PEG (150g l–1) in the simplified nutrient solution (pH 4.5). These 10 phosphoprotein spots are shown in the Pro-Q DPS-stained 2D-IEF/SDS–PAGE gels of Fig. 4 and in Supplementary Fig. S4. Spot no. relates to the corresponding spot number of Fig. 4 and Supplementary Fig. S4. FC, fold change (the relative protein spot volume of +PEG treatment to the relative protein spot volume of –PEG treatment). The proteins were identified by nano LC-MS/MS and BLASTed in NCBI database. See Supplementary Table S1 at JXB online for further details. MW, molecular weight.
| Spot no. | Identity | MW (Da) | GenBank acc. no. | FC |
|---|---|---|---|---|
| 1 | Ser/Thr-specific protein phosphatase 2A A regulatory subunit β isoform (Medicago sativa subsp.×varia) | 65161.6 | AAG29594 | 0.647 |
| 2 | Pyruvate kinase (Lactuca sativa) | 56174.9 | ABS87384 | 0.481 |
| 3 | Branched-chain amino acid aminotransferase, putative (Ricinus communis) | 45159.9 | XP_002530599 | 0.541 |
| 4 | Glucose-6-phosphate isomerase (Zea mays) | 68403.5 | NP_001147983 | 1.837 |
| 5 | Actin (Phaseolus acutifolius) | 33121.3 | AAZ95077 | 2.277 |
| 6 | Dehydrin (Phaseolus vulgaris) | 22955.6 | AAB00554 | 2.048 |
| 7 | Dehydrin (Phaseolus vulgaris) | 22955.6 | AAB00554 | 1.846 |
| 8 | Lactoylglutathione lyase, putative/glyoxalase I, putative (Arabidopsis thaliana) | 20830.4 | NP_001030996 | 2.414 |
| 9 | Dehydrin (Phaseolus vulgaris) | 22955.6 | AAB00554 | 2.438 |
| 10 | Dehydrin (Phaseolus vulgaris) | 22955.6 | AAB00554 | 1.625 |