Fig. 4.

Interaction of D-type cyclins with CDKs during maize germination. Antibodies against cyclins D2;2, 4;2, and 5;3 were used for immunoprecipitation and identification of the associated CDK in protein extracts from 0, 6, 12, 18, and 24 h-germinated axes. (A, B) Co-immunoprecipitation of CycD2;2 with CDKA and CDKB1;1 respectively; (D, E) co-immunoprecipitation of CycD4;2 with CDKA and CDKB1;1 respectively; (G, H) co-immunoprecipitation of CycD5;3 with CDKA and CDKB1;1 respectively. In (H) the intensity of the band at 18h was given a value of 1 as it could not be referred to the null value at time 0; thus, the band at 24h was compared to that at 18h. (C, F, I) Target proteins of the corresponding immunoprecipitating antibodies. Heavy chain IgGs were used as a loading control. Densitometry analysis was performed relating band intensity of all samples to the intensity of the loading control and then to the dry seed band. Each bar represents the mean±SE from three independent experiments. *Statistically significant value (P<0.001) compared to control.