Fig. 3.

RNA in situ hybridization analysis. (A–E and G) Longitudinal sections of soybean shoot apexes hybridized with antisense digoxigenin-labelled RNA as indicated. (A and B) Spatial expression of GmCLV3a and GmCLV3b are very similar, beneath the fourth layer of the meristem albeit a weaker expression for GmCLV3a. (C and D) No signals were detected for either GmCLV1a or GmCLV1b. (E) GmWUS is expressed beneath the fifth outermost layer of the meristem. (F) A longitudinal section of soybean shoot apical meristem stained with toluidine blue with the four distinct zonal layers indicated; the expression domain of GmCLV3 is false-coloured green (approximately 9 cells in total) while that of GmWUS red and the overlapping region yellow. (G) GmLOG1 transcripts are found with the L2–L4 layer. The inset shows a close up of its expression in the boundary region between the SAM and the developing leaf primordia. Bar, 0 μm (this figure is available in colour at JXB online).