Figure 7. Involvement of the adenylyl cyclase encoded by MSMEG_4279 in the oleic acid-induced resuscitation of NC cells.

NC cells were obtained and resuscitated in batch mode. In part A, the OD₆₀₀ was measured after resuscitation for 5 d for the wild type and the complemented strain, ΔAC(pMindAc), and after 11 d for the ΔAC knock-out strain. Oleic acid and cAMP were added at concentrations of 3.5 µM and 3 mM, respectively. In part B, samples were taken at intervals over the first four days from cultures of the wild type and the mutant, both with and without oleic acid, for the estimation of cellular metabolic activity (uracil incorporation). Asterisks in Fig. 6A indicate that the results are significantly different from the control for each strain by Student’s t-test. Asterisks in Fig. 6B indicate significant difference between wt cells and wt+oleic acid by Student’s t-test.