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. Author manuscript; available in PMC: 2013 Dec 24.
Published in final edited form as: Nature. 2008 Dec 7;457(7230):745–748. doi: 10.1038/nature07581

Figure 1. Assay for triggering of RAD51 disassembly.

Figure 1

a, Schematic of the multi-channel flow cell. After capturing RAD51-bound dsDNA molecules by one or both ends using optical traps, ATP hydrolysis is triggered by moving the complex to a Mg2+-containing channel, setting off disassembly. b, Kymograph of a RAD51-coated dsDNA molecule held from one end, stretched by flow. RAD51 patches are marked red in the cartoon on the left. Mg2+-induced filament disassembly (vertical arrow) is evidenced by simultaneous DNA contraction and a steady decrease in fluorescence (bottom graph).