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. Author manuscript; available in PMC: 2013 Dec 24.
Published in final edited form as: Oncogene. 2012 Dec 17;32(42):10.1038/onc.2012.530. doi: 10.1038/onc.2012.530

Figure 7.

Figure 7

pY654-β-catenin/HIF1α and p-c-Met accumulation, EMT markers, and tumor invasiveness induced by anti-VEGF are blocked by ROS inhibition. (a) Confocal images of anti-VEGF treated-RIP-Tag2 tumors ± NAC for 5 days stained for insulin (tumor cells, green) and E-cadherin (acinar cells and tumor cells, red). Scale bar, 200 µm. (b) Combination (anti-VEGF+NAC) or anti-VEGF alone (Anti-VEGF)-treated RIP-Tag2 tumor lysates were immunoprecipitated for pY654-β-catenin and blotted for β-catenin. (c–e) The above lysates were blotted for E-cadherin, N-cadherin, vimentin, Snail1, Twist, HIF1α, and β-actin (c) or pY416-Src, Src, pY845-EGFR, and EGFR (d) or immunoprecipitated with p-c-Met and blotted for c-Met (e).