TABLE 2.
Most Concordant ToxCast Assays for Each ZF Endpoint
| Zebrafish Endpoint | Concordance Count | ToxCast assay | Description | Assay Target |
|---|---|---|---|---|
| MORT | 94 | ATG_PXRE_CIS | PXR response element transcription | Xenobiotic response genes |
| YSE_ | 53 | |||
| AXIS | 47 | |||
| EYE_ | 31 | |||
| SNOU | 43 | |||
| JAW_ | 42 | |||
| OTIC | 17 | |||
| PE__ | 46 | |||
| SOMI | 14 | |||
| PFIN | 22 | |||
| SWIM | 15 | |||
| TR__ | 30 | |||
| ANY_ZF_ENDPOINT | 130 | |||
| ANY_ZF_ENDPOINT_EXCEPT MORTALITY | 76 | |||
| BRAI | 21 | ATG_PXRE_CIS | PXR response element transcription | Xenobiotic response genes |
| CLM_CellLoss_72hr | Cell loss | Cell loss | ||
| CFIN | 20 | CLZD_CYP2B6_6 | Expression of Cyp2B6 | Drug metabolism |
| PIG_ | 13 | ATG_PXRE_CIS | PXR response element transcription | Xenobiotic response genes |
| CLM_CellLoss_72hr | Cell loss | Cell loss | ||
| CLZD_CYP2B6 | Expression of Cyp2B6 | Drug metabolism | ||
| CIRC | 8 | NVS_ADME_hCYP2C19 | Inhibition of Cyp2C19 | Drug metabolism |
| TRUN | 22 | CLZD_CYP2B6_24 | Expression of Cyp2B6 | Drug metabolism |
| NC__ | 5 | BSK_BE3C_uPAR_up | ↑ in uPAR expression | Collagen/plasmin formation and immune and inflammatory responses |
| BSK_hDFCGF_CollagenIII_down | ↓ in CollagenIII expression | |||
| BSK_hDFCGF_PAI1_down | ↓ in PAI1 expression | |||
| BSK_SAg_CD40_down | ↓ in CD40 expression | |||
| BSK_SM3C_Proliferation_down | ↓ in proliferation |
Notes. Eighteen specific endpoints and 2 aggregate (ANY_ZF_ENDPOINT and ANY_ZF_ENDPOINT_EXCEPT_MORALITY) were statistically evaluated for concordance using Cohen’s kappa test for all in vivo ToxCast assays. The highest concordance count for each endpoint-ToxCast in vitro assay is illustrated along with the assay target.