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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Methods. 2013 Jun 25;66(3):370–379. doi: 10.1016/j.ymeth.2013.06.015

Figure 3. Setup for whole-mount immunohistochemistry (IHC), cryosection IHC and BABB preparation.

Figure 3

For whole-mount IHC, the entire procedure is executed in a nail polish slide well (a). For cryosection IHC, sections on coverslips are incubated on rubber stoppers in a moist covered chamber (b). For BABB preparation of the somitic tissue, the isopropanol washes are carried out in parafilm wells (c, d).