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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Methods. 2013 Jun 25;66(3):370–379. doi: 10.1016/j.ymeth.2013.06.015

Table 1. Some primary antibodies against sarcomeric proteins that cross-react with Xenopus orthologues.

In most cases the concentrations of the antibodies are not given, thus only the working dilutions for whole mount IHC are provided.

Antibody Species Supplier &
Catalog No#
Final
Dilution
Reference
Anti-α-actinin EA-53 Mouse monoclonal Sigma A7811 1:200 [33]
Anti-α-cardiac actin Ac1-20.4.2 Mouse monoclonal American Research Products 03-61075 1:400 [34]
Anti-pan α-actin Rabbit polyclonal Sigma A 2103 1:200 [35]
Anti-actin 5C5 Mouse monoclonal Sigma A2172 1:500 [27]
Anti-α-skeletal actin JLA20 Mouse monoclonal Developmental Studies Hybridoma Bank unknown [28]
Anti-α-tropomyosin TM311 Mouse monoclonal Sigma T 2780 1:200 [36]
Anti-tropomyosin CH1 Mouse monoclonal Developmental Studies Hybridoma Bank 1:50 [9]
Anti-leiomodin Rabbit polyclonal Proteintech 14948-1-AP 1:400
Anti-titin 9D10 Mouse monoclonal Developmental Studies Hybridoma Bank unknown [6]
Anti-myosin F59 Mouse monoclonal Developmental Studies Hybridoma Bank 1:50 [6]
Anti-myosin MF20 Mouse monoclonal Developmental Studies Hybridoma Bank 1:1 [29]
Anti-myosin IIa 56-396-5 Mouse monoclonal Developmental Studies Hybridoma Bank 1:300 [30]
Anti-cofilin2 Rabbit polyclonal Millipore 07-300 1:300 [31]
Anti-phospho cofilin1 Rabbit polyclonal Santa Cruz SC-12912-R 1:3000 [31]
Anti-phospho cofilin2 Rabbit polyclonal Abcam AB14134 1:300 [31]
Anti-desmin DB-E-5 Mouse monoclonal Millipore MAB3430 1:200 [32]
Anti-cardiac troponin T CT3 Mouse monoclonal Developmental Studies Hybridoma Bank 1:10 [9]
Anti-capZ 1E5.25 Rabbit polyclonal Developmental Studies Hybridoma Bank unknown [6]