FIGURE 6.

Chronic administration of RS 67333 prevents object recognition memory loss in 5XFAD mice. Two-month-old 5XFAD females (Tg) and WT littermates were treated with RS 67333 or vehicle according to protocol 2 (1 mg/kg, twice a week for 2 months) and then recognition memory was assessed with the novel object recognition (NOR) test. Habituation sessions were started 3 days after the end of treatment and the interval between training session and test was of 1 h. (A) RS 67333-treatment reverses cognitive impairment as indicated by the higher percentage of time devoted to the exploration of the novel object in comparison to mice treated with vehicle. Data are the mean ± SEM (n = 6–7 mice/group). ^*p < 0.05, ^**p < 0.01 versus familiar object (two-way ANOVA followed by Bonferroni’s test). (B) This finding was confirmed also by calculating the discrimination index [(novel - familiar)/(familiar + novel)]. Data are the mean ± SEM. Indices different from zero: ^*p < 0.05, ^**p < 0.01 (unpaired Student’s t test). ^§p < 0.05 versus Tg-vehicle group (two-way ANOVA followed by Tukey’s test). (C) The total exploration time was not significantly different among groups.