Skip to main content
. 2013 Dec 9;2013:485213. doi: 10.1155/2013/485213

Figure 4.

Figure 4

Reductions in the levels of IL-17 and IFN-γ in the lacrimal glands following immunization with the M3R208–227 peptide. To further observe whether the concentrations of cytokines decreased in the lacrimal glands, we also examined the production of IFN-γ, IL-4, and IL-17 in the lacrimal glands of NOD/LtJ mice by immunofluorescence staining. Whole lacrimal glands from NOD/LtJ mice were removed, fixed, embedded, and incubated with primary antibodies, followed by a secondary goat anti-rat IgG antibody. The results of immunofluorescence staining demonstrated that the production of IL-17 and IFN-γ decreased in the lacrimal glands of NOD/LtJ mice immunized with the M3R208–227 peptide. In contrast, the production of IL-4 was not observed in the lacrimal glands of NOD/LtJ mice in all groups.