Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Exp Eye Res. 2013 Oct 17;116:10.1016/j.exer.2013.10.007. doi: 10.1016/j.exer.2013.10.007

Fig. 1. Flow cytometry analysis of stability and percentage of chimerization achieved by bone marrow transfer.

Fig. 1

Peripheral blood preparations from WT (negative control), GFP+ transgenic (positive control) and chimeric mice were stained with propidium iodide and gated on live cell populations. Live cells were further analyzed on an SSC vs. FL1 (GFP+) plot. The upper panel shows the absence of GFP+ staining in peripheral blood from WT mice (left upper panel) and > 99% GFP+ staining in peripheral blood from GFP+ transgenics (GFP-transgenic C57/BL/6-Tg(UBC-GFP)30 Scha/J). The lower panel shows percentage of chimerization achieved in WT mice at 15 days after tail vein injection (left lower panel), at one month after tail vein injection (center lower panel) and 2 months after tail vein injection (right lower panel) of GFP+ bone marrow. Data shown is representative of 51 chimeras.