Fig. 2.

GFP+SMA+ immunohistochemistry images of corneal sections from GFP+ chimeric mice at one month after irregular PTK. In each row, the left lane shows GFP stained green; the middle lane shows SMA+ cells stained red; and the right lane is an overlay of DAPI, GFP and SMA staining. A: is immunohistochemistry in the cornea of a chimeric mouse at one month after irregular PTK where the primary antibodies were omitted (controls). B is immunohistochemistry for SMA and GFP proteins in the cornea of a chimeric mouse at one month after irregular PTK. The arrows indicate representative SMA+GFP+ positive cells that are myofibroblasts that differentiated in the cornea from bone marrow-derived cells. The arrowheads are SMA+ myofibroblasts that are GFP-, indicating they likely differentiated from cells derived from keratocytes in the cornea, although it cannot be excluded that one or both of these cells also differentiated from bone marrow-derived cells since it is not possible to achieve 100% chimerism. This section was selected to show SMA+ cells that are both GFP- and GFP+. When counts were performed in many fields on many sections in several corneas at one month after irregular PTK in chimeric mice, more than 90% of SMA+ cells were GFP+ (Barbosa, et al., 2010).