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. 2013 Dec 24;4:526. doi: 10.3389/fpls.2013.00526

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Copyright © 2013 Nyaboga, Njiru, Nguu, Gruissem, Vanderschuren and Tripathi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Agrobacterium-mediated transformation of cassava cultivar Serere using FEC. (A) In vitro shoot culture; (B) axillary bud (arrow); (C) primary somatic embryos; (D) friable embryogenic callus; (E) Agrobacterium-inoculated FEC proliferating on selective medium and transient GUS assay (upper right corner); (F) somatic embryos/cotyledons on selective embryo development and maturation medium; (G) mature somatic embryos on shoot elongation medium and GUS positive somatic embryos (up); (H) shoots developing on shoot elongation medium; (I) transgenic plantlets germinated on basic cassava medium and GUS expression in shoot (left).