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A: relative Fgf 2 mRNA expression by RT-PCR in ischemic and nonischemic (contralateral) hindlimbs of Fgf 2+/+ mice. Shown are representative results at 3, 7, and 14 days after left femoral artery ligation. Aliquots of the amplification reaction were removed during sequential cycles and gel electrophoresed. Gapdh serves as control for cDNA template quantity and linear amplification. B: no Fgf 2 mRNA was detected in tissues from Fgf 2−/− mice with RT-PCR.
