HEK293 cells transfected with huNOD2 or empty vector and NF-κB-ELAM firefly luciferase and control thymidine kinase Renilla luciferase were stimulated overnight with media, MDP, or heat-killed B. pseudomallei 1026b at various bacteria:cell ratios. Cells were then lysed and NF-κB activation determined by relative light emission (firefly/Renilla luciferases). Data displayed are means ± SD of triplicate conditions normalized to empty vector media values. The data shown are from one of six representative and independent experiments. *, p≤0.05; **, p≤0.01; ***, p≤0.001.