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. 2013 Dec 9;2013:326041. doi: 10.1155/2013/326041

Figure 1.

Figure 1

Involvement of PAR1/PAR3 receptor in thrombin-induced MMP-13 expression in human chondrocytes (a) qPCR analyses of MMP-13 mRNA and (b) and (c) ELISA and Western blotting analyses of protein expression of MMP-13 in chondrocytes incubated with thrombin for 24 h. (d) and (e) qPCR and ELISA analyses of MMP-13 expression in chondrocytes treated with thrombin alone (3 U/mL), thrombin + PPACK (30 nM), SFLLRN-NH2 (100 μM), thrombin + TFRGAP-NH2 (100 μM), or thrombin + GYPGQV-NH2 (100 μM) for 24 h. (f) and (g) qPCR and ELISA analyses of MMP-13 expression in chondrocytes, which were transfected with siRNAs against PAR1, PAR3, PAR4, or control siRNA for 24 h and then stimulated with thrombin for 24 h. *P < 0.05 as compared with basal level. # P < 0.05 as compared to the levels in the thrombin-treated group.