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. Author manuscript; available in PMC: 2014 Dec 25.
Published in final edited form as: Chem Commun (Camb). 2013 Dec 25;49(99):11650–11652. doi: 10.1039/c3cc47022a

Table 1.

Rates (Vmax) and apparent KM values for formation of IN-NAD in a biomimetic assay. a

KatG/mutants Vmax K M kcat/KM
(μM/min) (μM) (mM−1min−1)
WT 1.52 ± 0.02 192 ± 8 7.9
Asp137Ser 1.12 ± 0.02 17.5 ± 1 64
Ser315Thr 0.16 ± 0.01 8400 ± 700 0.02
Arg418Leu 0.76 ± 0.02 165 ± 16 4.6
Tyr229Phe 1.03 ± 0.03 101 ± 19 10.2
Trp321Phe 1.30 ± 0.02 227 ± 13 5.7
a

KatG (1 μM), NAD ( 200 μM), H2O2 (3.0 μM/min), and varying INH.