Figure 3.

Disrupting TfAP-2 or Twz expression in Tdc2-specific octopaminergic neurons affects male behavior. TfAP-2 and Twz RNAi were expressed specifically in octopaminergic neurons using the Tdc2-GAL4 driver. (A) Total interactions that were either high- or low-intensity aggression or courtship behavior for all genotypes were determined. All males were between 5 and 7 days old. The types of behaviors were distributed into three catagories—HIF, LIF, and courtship behavior—and the number of each type of behavior performed is represented. In all instances, the assay was repeated at least 10 times (n = 20 males/treatment; *P < 0.05, **P < 0.005 compared with controls, two-way ANOVA with Bonferroni post hoc test for multiple comparisons). (B–D) Mating behavior of 5- to 7-day-old males toward 3- to 4-day-old Csorc wild-type virgin female was recorded over a 10-min period or until copulation occurred. In all instances the assay was repeated at least 10 times. (B) Male courtship behavior toward a virgin female. (C) Total number of behaviors a male performed. (D) Amount of time, in seconds, before the first courtship behavior, or latency, was determined. Percentage of time a male spent actively courting (courtship index) was determined. In B and D, different letters indicate similar groups (i.e., “a” is significantly different than “b” or “c” and so on). n = 20 males per aggression or mating assay; *P<0.05 **P < 0.005 compared with controls, one-way ANOVA with Bonferroni post hoc test for multiple comparisons.