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. 2013 Oct 18;196(1):349–362. doi: 10.1534/genetics.113.158402

Figure 5.

Figure 5

TfAP-2 and Twz regulate genes involved in octopamine signaling. (A) Simplified schematic diagram of the pathway involved in octopamine production and secretion. (B–D) The transcript levels of genes (A) Tdc2, (B) Tbh, and (C) Vmat. RNA was collected from the heads of 5- to 7-day-old males for each genotype. qPCR was repeated at least seven times for each transcript. Tbh and Vmat transcript levels are significantly lower in flies where TfAP-2 or Twz are knocked down in octopaminergic neurons (one-way ANOVA, P < 0.005), while their transcript levels are significantly increased when TfAP-2 is overexpressed in these same neurons (n = 7 qPCR runs; *P < 0.05 **P < 0.005 compared with controls, one-way ANOVA with Bonferroni post hoc test for multiple comparisons). In C and D different letters indicate similar groups (i.e., ‘a’ is significantly different than ‘b’ or ‘c’ and so on).