FIGURE 3.

Leptin signaling is required for normal rates of glucose uptake, glycolysis, and mitochondrial respiration in activated T cells. (A and B) CD4+ T cells were isolated from db+/+ (black bar) or db−/− (white bar) mice. Glucose uptake was assessed by ³[H]-2-DG uptake (A) and glycolytic flux was determined by the generation of tritiated water by enolase (B) in both resting cells and cells activated with anti-CD3 and anti-CD28 antibodies for 48 hours, as indicated. Representative data are shown. (C and D) Extracellular acidification rates (C) and basal oxygen consumption rates (D) were measured using a Seahorse Extracellular Flux Analyzer in activated T cells isolated from wildtype (db+/+; black bar) or leptin receptor-deficient (db−/−; white bar) mice. Data are representative of two independent experiments.