Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Immunol. 2013 Nov 22;192(1):10.4049/jimmunol.1301158. doi: 10.4049/jimmunol.1301158

FIGURE 5.

FIGURE 5

The effects of leptin on activated T cell function and metabolism are hematopoietic cell intrinsic. Irradiated Rag recipient mice were transplanted with bone marrow isolated from wildtype (db+/+; black bars) or leptin receptor-deficient (db−/−; white bars) donors. Following full immune reconstitution at 18 weeks, the following experiments were performed. (A) CD4+ T cells were isolated and activated with anti-CD3 and anti-CD28 for 48 hours, followed by measurement of IFN-γ production by ELISA. (B and C) Resting and activated CD4+ T cells were evaluated for glucose uptake; data are representative of five independent experiments (B) and glycolytic flux; data are representative of three independent experiments (C).