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. Author manuscript; available in PMC: 2014 Dec 19.
Published in final edited form as: Chem Biol. 2013 Nov 7;20(12):10.1016/j.chembiol.2013.09.020. doi: 10.1016/j.chembiol.2013.09.020

Figure 3.

Figure 3

SAHA increases the protein and mRNA level of BiP without an apparent induction of the XBP1 splicing.

(A and B) SAHA (2.5 μM, 24 h) only increases the protein level of BiP among tested major ER and cytosolic chaperones and ERAD factors in HEK293 cells stably expressing WT α1β2γ2 or α1(A322D)β2γ2 GABAa receptors (n = 3) (A). Quantification is shown in (B).

(C) AHA (2.5 μM) increases the mRNA level of BiP using quantitative RT-PCR analysis.

(D) SAHA (2.5 μM) does not induce the splicing of XBP1 using RT-PCR analysis. Thapsigargin (Tg) is used as a positive control to induce XBP1 splicing. GAPDH is used as a loading control. Each data point in (B) and (C) is reported as mean ± SEM. * p < 0.05 See also Figure S3 and Table S1.