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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Alcohol Clin Exp Res. 2013 Aug 22;38(2):384–391. doi: 10.1111/acer.12244

Figure 1.

Figure 1

Effect of ethanol on CCL2, IL-6, and TNF-α expression in adult and adolescent mouse hippocampus. Animals were given 6.0 g/kg/day ethanol split into two doses and administered 7 h apart. Ethanol was administered for 10 days and mice were sacrificed 24h after the final dose of ethanol. Water was administered to vehicle mice. The hippocampus was isolated, RNA prepared, cDNA synthesized, and mRNA levels were determined by real-time quantitative RT-PCR. Results are expressed as fold changes relative to vehicle treated mice, and all values are normalized against β-actin. Values are mean +/− SEM from 10 vehicle and 13 ethanol treated adult mice (A) or from 8 vehicle and 6 ethanol treated adolescent mice (B). Duplicate PCR reactions were performed on each sample. Open bars represent vehicle-treated mice. Solid bars represent ethanol-treated mice. ***, P<0.001 versus vehicle treated mice.