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. Author manuscript; available in PMC: 2015 Jan 3.
Published in final edited form as: Vaccine. 2013 Sep 16;32(2):10.1016/j.vaccine.2013.09.004. doi: 10.1016/j.vaccine.2013.09.004

Figure 2. Detection of serum IgG antibody to Ec, Fn or Pg bacteria and to Pg extracts by ELISA.

Figure 2

Three weeks and six weeks after immunization, rat sera were collected and reacted with ELISA plates coated with (A) Ec bacteria, (B) Fn bacteria or (C) Pg bacteria. (D) Rat sera from six weeks post immunization were also reacted with ELISA plates coated with Pg extracts as described in “Materials and Methods”. Each experiment was performed in duplicate and readings were recorded by a microplate reader at 405nm. (Mean ± SE, **p< 0.01)