Fig. 2.
Increased ROS in hematopoietic cell lines expressing an NHD13 transgene. (A) Ba/F3 cells stably transfected with pEF1a-NHD13 construct (BaF3/pNHD13) showed higher ROS level than cells transfected with empty vector (BaF3/pEF1a) or Ba/F3 cells. (B) Elevated ROS level was observed in 32D/ pEF1a-NHD13 (32D/pNHD13) cells compared with 32D/pEF1a and 32D cells. Bar graphs and statistics were acquired from stable transfectants at 3 different passages. The error bars represent SEM. *, p<0.05; **, p<0.01