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. 2013 Dec 18;7:23–32. doi: 10.2147/OTT.S54575

Figure 4.

Figure 4

Knockdown of Pur-beta prevents T-oligo-induced upregulation of senescence-associated proteins in MU melanoma cells.

Note: (A) Pur-beta-deficient MU cells and control shRNA-transfected MU cells were treated with T-oligo for 24 hours. Pur-beta shRNA prevented T-oligo-induced upregulation of E2F1 (6.5-fold) and p53 (2.0-fold) expression, and phosphorylation of p53(ser15) (6.0-fold) in MU cells, when compared with control-transfected MU cells treated with T-oligo. (B) The hypophosphorylation of pRb (2.0-fold) and upregulation of p21 (1.7-fold) seen in control-transfected MU cells, were also prevented in Pur-beta-deficient cells treated with T-oligo.

Abbreviations: Pur, purine-rich element binding protein; shRNA, short hairpin ribonucleic acid; Pur-β sh, Pur-β shRNA; T, T-oligo; C, complementary oligonucleotide; p-p53, phospho-p53.