Table S1.

List of proteins identified by mass spectrometry

Symbol	Protein name	Protein accession numbers	Function	Protein identification probability	Number of unique peptides	Percentage sequence coverage
HNRNPDa	Isoform 1 of heterogeneous nuclear ribonucleoprotein D0	IPI00028888	Increases senescence1	99.00%	2	6.76%
HNRNPA2B1a	Isoform B1 of heterogeneous nuclear ribonucleoproteins A2/B1	IPI00396378	Increases cell proliferation2	99.00%	2	6.80%
PURBb	Transcriptional activator protein Pur-beta	IPI00045051	Increases senescence and apoptosis3	100.00%	10	21.50%
MSI1a	RNA-binding protein Musashi homolog 1	IPI00001007	Inhibits p21 and increases apoptosis4	99.00%	2	7.18%
HNRNPCb	Isoform C1 of heterogeneous nuclear ribonucleoproteins C1/C2	IPI00216592	Upregulates p53 activity5	99.90%	3	18.81%
HNRNPA1a	Isoform A1-B of heterogeneous nuclear ribonucleoprotein A1	IPI00215965	Increases apoptosis6	100.00%	9	25.50%
HNRNPDa	Isoform 4 of heterogeneous nuclear ribonucleoprotein D0	IPI00220685	Increases senescence1	99.00%	2	30.00%
GNAI2a	Isoform 2 of guanine nucleotide-binding protein G(i), alpha-2 subunit	IPI00217906	Decreases shielding from apoptosis7	99.90%	3	10.00%
VDAC2a	Voltage-dependent anion-selective channel protein 2	IPI00024145	Increases apoptosis due to reduced inhibition of BAK8	99.50%	3	10.10%
VDAC1a	Voltage-dependent anion-selective channel protein 1	IPI00216308	Increases apoptosis9	99.90%	3	12.00%

Notes: List of the potential T-oligo binding proteins identified by mass spectrometry. Protein bands present (∼50–37 kDa) after treatment with T-oligo were cut from the gel and analyzed by mass spectroscopy using ABI 4700 MALDI-TOF/TOF MS unit. For protein identification, at least two peptides per protein with 90% peptide confidence and 90% protein confidence thresholds were used. Functions of the identified proteins are based on Uniprot and PubMed information. Proteins which have a role in DNA damage responses, or are known to bind telomeric DNA, such as hnRNP A1, A2–B1, and D, are shown.

^aProteins which are important and need to be further studied

^bproteins which may have a role in T-oligo-induced DNA damage responses.

Abbreviations: Pur, purine-rich element binding protein; DNA, deoxyribonucleic acid; hnRNP, heterogeneous nuclear ribonucleoprotein; MALDI-TOF/TOF MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.