Figure 7.

Stimulation of eIF4F complex assembly and modification of eIF4E and 4E-BP-1 in HSV-1-infected cells depends on the ICP0 protein. NHDF cells were either mock infected (M), infected (MOI = 3) with the ICP0 null mutant 7134, or infected with a virus in which the ICP0 mutation was repaired (7134R). After 8 h, total protein was isolated and fractionated by SDS-PAGE or IEF, and analyzed by immunoblotting with the indicated antibodies. (A) 4E-BP1 is not degraded in NHDF cells infected with an ICP0 deletion mutant. (B) eIF4E and 4E-BP1 are not phosphorylated in cells infected with an ICP0 mutant, and p38 is not activated. (C) Primary human fibroblasts (NHDF cells) were growth arrested by serum starvation and either mock infected or infected with 7134 or 7134R. eIF4F complexes were isolated by adsorption to 7-methyl GTP Sepharose and analyzed as described in the legend to Figure 4.