Table 2.
Strains and plasmids
| Strain | Relevant genotype or description | Reference or source |
|---|---|---|
| Caulobacter crescentus | ||
| CB15N | Synchronizable variant strain of CB15 | Evinger and Agabian 1977 |
| UJ284 | CB15N ΔpleD | Aldridge et al. 2003 |
| UJ417 | CB15N and plasmid pPA41 | Aldridge et al. 2003 |
| UJ506 | CB15N ΔpleC | Aldridge et al. 2003 |
| UJ626 | UJ284 and plasmid pPA53-4 | This study |
| UJ627 | CB15N and plasmid pPA53-4 | This study |
| UJ998 | CB15N divJ::Ω | Aldridge et al. 2003 |
| UJ1000 | CB15N ΔpleC::Ω | Aldridge et al. 2003 |
| UJ1168 | CB15N and plasmid pPA114-47 | Aldridge et al. 2003 |
| UJ1169 | UJ284 and plasmid pPA114-47 | Aldridge et al. 2003 |
| UJ1420 | UJ1000 and plasmid pPA53-4 | This study |
| UJ1466 | UJ998 and plasmid pPA53-4 | This study |
| UJ1875 | UJ506 and plasmid pPA534 | This study |
| UJ1909 | CB15N and plasmid pSW6 | This study |
| UJ1910 | UJ284 and plasmid pSW7 | This study |
| UJ2222 | UJ506 and plasmid pSW7 | This study |
| UJ2223 | UJ998 and plasmid pSW7 | This study |
| UJ2224 | UJ1000 and plasmid pSW7 | This study |
| UJ2262 | UJ284 and plasmid pSW8 | This study |
| Escherichia coli | ||
| DH10B | F−mcrA Δ(mrr− hsd RMS−mcrBC) ϕ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara, leu)7697 araD139 galU galK nupG rpsL thi pro hsdR− hsd+ recA RP4-2-Tc::Mu-Tn7 | Simon et al. 1983 |
| BL21 (DE3) | E. coliB F−dcm ompT hsdS(rB−mB−) | Stratagene |
| pLysS | gal λ(DE3) [pLysS CAMr] | |
| BL21-CodonPlus | E. coli B F−ompT hsdS(rB− mB−) dcm+ | Stratagene |
| (DE3)-RIL | Tetrgal 1 (DE3) endA Hte [argU ileY leuW Camr] |
| Plasmid | Relevant genotype or description | Reference or source |
|---|---|---|
| pMR20 | TetR low copy number vector | Roberts et al. 1996 |
| pBAD | AmpR expression plasmid | Invitrogen |
| pGEX4T3 | AmpR vector for creation of GFP-fusion proteins | Amersham Biotech |
| pEGFP-N1 | AmpR expression plasmid | Clontech |
| pET11 | AmpR expression plasmid | Stratagene |
| pPA53-4 | pMR20; pleD-GFP under the control of divK promoter | This study |
| pSW6 | pMR20; pleDD53N-GFP under the control of divK promoter | This study |
| pSW7 | pMR20; pleD*D53N-GFP under the control of divK promoter | This study |
| pSW8 | pMR20; pleDGG368DE-GFP under the control of divK promoter | This study |
| pPA69 | pGEX4T3; pleD, N-terminal GST-tag | This study |
| pRP49 | pBAD; pleC, N-terminal His6 tag | This study |
| pRP63 | pBAD; divJ, N-terminal His6 tag | This study |
| pCC2 | pET11; pleD, C-terminal His6 tag | This study |
| pRP87 | pET11; pleDD53N, C-terminal His6 tag | This study |
| pRP88 | pET11; pleDΔ368-372, C-terminal His6 tag | This study |
| pRP89 | pET11; pleD*, C-terminal His6 tag | This study |
| pRP90 | pET11; pleD*D53N, C-terminal His6 tag | This study |
| pRP91 | pET11; pleDGG368DE, C-terminal His6 tag | This study |