Figure 6.
GM-CSF adaptive immune modulation attenuates innate immune activation. Photomicrographs and enumeration of anti-CD11b+ microglia in the SN of mice treated with PBS, 4 doses of 16 mg MPTP/kg alone, and MPTP mice receiving donor spleen cells enriched for either CD4+ T cells or Treg from mice treated with 5 daily GM-CSF doses of 50 μg/kg. Midbrain tissues were obtained 2 days after MPTP-intoxication. A. Midbrain sections stained with anti-CD11b Ab, HRP-conjugated secondary Ab, and visualized with DAB. Activated microglia are identified as those stained cells exhibiting large amoeboid cell bodies and shortened processes. B. Differences in mean ± SD (n = 5 per group) were determined where p < 0.05 compared with groups treated with aPBS, bMPTP alone, and MPTP intoxicated receiving either cCD4+ T cells (CD4) or dTreg.