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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: J Neuroimmunol. 2013 Oct 29;265(0):10.1016/j.jneuroim.2013.10.009. doi: 10.1016/j.jneuroim.2013.10.009

Figure 7.

Figure 7

GM-CSF induces both pro- and anti-inflammatory gene expression changes. All TH-GFP/21-31 mice that express GFP under control of the TH promoter were treated with MPTP. Controls received no other treatment, one group was pretreated with 50 μg GM-CSF/kg 5 days before MPTP intoxication (4 doses of 16 mg/kg), and another group received GM-CSF-induced Treg by adoptive transfer within 12 hours after MPTP intoxication. A. Laser capture microdissection was used to isolate SN tissues harvested 2 days after MPTP intoxication and mRNA from each animal was isolated. Shown is the demarcation of the SN by the presence of GFP-TH neurons (top panel) and a representative section to be dissected (bottom panel). B. qRT-PCR data showing gene expression changes within the SN of mice pretreated with GM-CSF followed by MPTP (GM-CSF Pretreatment) or GM-CSF-induced Treg compared to those of MPTP controls (n = 3 for each group). Fold changes and p value determined by SABioscience RT2 Profiler PCR Array Data Analysis, version 3.5.