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. Author manuscript; available in PMC: 2013 Dec 26.
Published in final edited form as: J Mol Cell Cardiol. 2009 Feb 26;46(6):10.1016/j.yjmcc.2009.02.015. doi: 10.1016/j.yjmcc.2009.02.015

Fig. 4.

Fig. 4

The effects of insulin treatment on mitochondria biogenesis and total myocardial complex activities in diabetic myocardium. (A) Mitochondria abundance. The abundance of mitochondria DNA was measured by quantitative real-time PCR to determine the copy number of ND5 and β-actin. 2 days after streptozotocin injection, the diabetic mice were treated with insulin (I) or Phlorizin (Ph) for 8 days when indicated. Data represent mean±SEM from 6 mice in each group. (B) Total myocardial complex V activities adjusted by the content of mitochondria. Total myocardial complex V activities per myocardial unit were calculated with the following formula: total complex V activity=(complex V activity per mitochondria unit)×(mitochondria content per myocardial unit). STZ-DM mice were treated with insulin (I) and phlorizin (Ph) when indicated. (**p<0.01 vs. STZ; #p<0.005 vs. control; ##p<0.001 vs. control; &p<0.0005 vs. control).