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. Author manuscript; available in PMC: 2013 Dec 26.
Published in final edited form as: J Mol Cell Cardiol. 2009 Feb 26;46(6):10.1016/j.yjmcc.2009.02.015. doi: 10.1016/j.yjmcc.2009.02.015

Fig. 7.

Fig. 7

In vivo inhibition of Akt signaling blunted insulin-stimulated complex V activities in myocardium. Mice were overnight-fasted and anesthetized. When indicated, LY294002 (Ly) were injected (40 mg/kg BW, i.p.) 20 min prior to insulin injection. Myocardium was harvested after insulin injection, mitochondria were isolated and mitochondria proteins were used for immunoblotting. Insulin-stimulated p-Akt translocation was inhibited by LY294002 in myocardium (upper panel). The effect of insulin on complex V activity was blunted in the LY294002-pretreated myocardium (lower panel). #p<0.005 vs. insulin-treated control, **p<0.01 vs. control basal.