Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 26;7:204. doi: 10.3389/fncir.2013.00204

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Ikrar, Guo, He, Besnard, Levinson, Hill, Lee, Hen, Xu and Sahay.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Mature dentate granule neurons in iBax^nestin mice show redistribution of inhibitory synaptic weights without changes in pattern of mIPSC generation. (A) Recording from a representative mature dentate granule cell. Left, top: A low magnification image showing a patch electrode on a neuron located in the outer blade. The area outlined by the white square is shown below in higher magnification. Right: The same neuron processed for biocytin, which was placed inside the recording pipette. Green: biocytin, Blue: DAPI. (B) No significant difference in the average mIPSC frequency (top left, Veh: 8.7 ± 0.89 Hz; TAM: 9.2 ± 1.03 Hz; t-test: p = 0.71) and the average mIPSC amplitude (bottom left, Veh: 45 ± 3.3 pA, n = 11; TAM: 42 ± 3.2 pA, n = 20; t-test: p = 0.57) between control and iBax^nestin mice. Top right: Representative mIPSC traces. Bottom right: Average mIPSC traces. There was no difference in the kinetics of the mIPSCs between the two groups (10–90 rise: Veh: 1.9 ± 0.07 ms; TAM: 1.9 ± 0.05 ms, p = 0.85; decay time constant: Veh: 6.1 ± 0.45 ms; TAM: 6.5 ± 0.28 ms, p = 0.41). (C) No change in pattern of mIPSC generation between two groups. Top: Cumulative probability of mIPSC interevent intervals from control and iBax^nestin mice. No significant difference between the two groups (Kolmogorov-Smirnov test: p = 0.1). Bottom: Subtraction of the two cumulative probability graphs (Veh–TAM) confirms a minimal change in the distribution of mIPSCs interevent intervals. (D) Changes in the distribution of inhibitory synaptic weight in iBax^nestin mice Top: Cumulative probability of mIPSC amplitudes from control and iBax^nestin mice. There was a significant difference between the two groups (Kolmogorov-Smirnov test: p < 0.001). Bottom: Subtraction of the two cumulative probability graphs (Veh–TAM) reveals a significant increase in the fraction of smaller and larger mIPSCs in the TAM treated group.