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. 2013 Dec 26;7:204. doi: 10.3389/fncir.2013.00204

Figure 6.

Figure 6

Inducible recombination of Bax in progenitors in adult DG increases the number of excitatory synaptic contacts of adult-born dentate granule neurons with hilar interneurons. (A,B) Representative images of DG of Bax +/+ and Bax f/f littermates processed for GAD67, GFP, and DAPI and quantification showing increased number of 4 weeks old GFP+ adult-born dentate granule neurons in Bax f/f mice. (C) Confocal images in xy, xz, and yz planes showing GFP+ contacts of small MFT with GAD67+ processes in hilus of Bax +/+ and Bax f/f mice. (D) DG of Bax f/f mice (140.7 ± 12.9) show a significant increase in GFP+ MFTs in hilus compared to Bax +/+ littermates (53.16 ± 7.24) (n = 12 hemisections from 2 Bax +/+ mice, n = 17 hemisections from 3 Bax f/f mice, p < 0.001, unpaired t-test). (E) Hilus of Bax f/f (2.55 ± 0.22) and Bax +/+ (2.22 ± 0.21) littermates show equivalent number of contacts with GAD67 processes per MFT (n = 75 MFTs from 2 Bax +/+ mice and n = 70 MFTs from 3 Bax f/f mice, p = 0.27, unpaired t-test). Scale bar: 100 μ m (A), 2 μ m (C). Results are Mean ± SE. *p <0.05.