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. 2013 Sep 16;591(Pt 23):5895–5911. doi: 10.1113/jphysiol.2013.260521

Figure 3. Old cardiomyocytes have mild mitochondrial uncoupling, which is exacerbated in the presence of ISO.

Figure 3

A, representative images of mitochondrial membrane potential at 0 s measured by using TMRE. B and C, traces and mean data of normalized TMRE fluorescence at baseline and ISO (30 nm). Data are normalized to the minimum TMRE fluorescence in the presence of electron transport chain uncoupler FCCP (50 μm): young, 5.71 ± 0.55; young + ISO, 5.51 ± 0.53; old, 4.16 ± 0.42; and old + ISO, 3.87 ± 0.41. *P < 0.05 for young vs. old and for young + ISO vs. old + ISO (unpaired analysis), ##P < 0.01 for young vs. young + ISO (paired analysis), and ###P < 0.001 for old vs. old + ISO (paired analysis). n= 20–21 cells from 2–4 heart preparations; all values are mean ± SEM.