Skip to main content
. 2013 Sep 16;591(Pt 23):5895–5911. doi: 10.1113/jphysiol.2013.260521

Figure 5. Mitochondrial ROS scavenger mito-TEMPO reverses the effects of ageing with regard to SR Ca2+ release under β-adrenergic stimulation.

Figure 5

A, confocal line scan images and corresponding temporal Fluo-3 F F0−1 profiles at 1 Hz from cardiomyocytes from young and old rabbits, and from cardiomyocytes from old rabbits pretreated with mito-TEMPO (MT, 25 μm) for 10 min. All experiments were performed in the presence of 30 nm ISO. B, mean data for Ca2+ transient amplitude: young, 4.23 ± 0.24; old, 2.98 ± 0.26; and old + MT, 3.80 ± 0.42 ΔF F0−1. *P < 0.05 for young vs. old. C, mean data for caffeine transient amplitude: young, 4.65 ± 0.27; old, 3.25 ± 0.27; and old + MT, 4.44 ± 0.41 ΔF F0−1. *P < 0.05 for old vs. old + MT, **P < 0.01 for young vs. old. D, mean data for kcaff decay rate constant: young, 0.57 ± 0.04; old, 0.42 ± 0.02; and old + MT, 0.66 ± 0.07 s−1. *P < 0.05 for young vs. old. **P < 0.01 for old vs. old + MT. E, mean data for kSR rate constant: young, 5.09 ± 0.29; old, 5.93 ± 0.58; old + MT, 4.48 ± 0.72 s−1. P= NS. n = 13–17 cells from 3 heart preparations; all values are mean ± SEM.