Figure 8. Scavenging of mitochondrial ROS restores decreased RyR free thiol content in ageing myocytes treated with the β-adrenergic agonist ISO.

A, representative mBB-fluorescence of RyRs from young, old, and old myocytes incubated with mito-TEMPO (25 μm, 10 min) and exposed to ISO (30 nm, 3 min) in the absence and presence of the oxidizing agent DTDP (0.2 mm) and in the presence of the reducing agent DTT (5 mm). B, relative thiol content of RyRs from young myocytes, old myocytes, and old myocytes treated with mito-TEMPO myocytes. *P < 0.05. n= 4–6 heart preparations; all values are mean ± SEM.