Figure 6. Cyanide sensitivity of TASK channels in type-1 cells.

A, B, representative single-channel recording showing the reversible inhibition of background channel activity by 2 mm NaCN in control type-1 cells. Recording conditions were as in Fig. 5. C, superimposition of all-points histograms derived from recordings made during superfusion with control and cyanide Tyrode solutions. D, comparison of relative background channel activity in cell-attached patches under control and cyanide exposure (n= 14 patches). E, F, single-channel recording from a Task-1^−/− type-1 cell showing the effects of cyanide. G, superimposed histograms constructed from Task-1^−/− data obtained during control and cyanide Tyrode superfusion. H, relative channel activity for Task-1^−/− during control and cyanide exposure (n= 6 patches). I, J, Task-3^−/− single-channel activity inhibition by cyanide. K, Superimposition of all-points histograms created from Task-3^−/− type-1 cell control and cyanide recordings. L, comparison of relative channel activity in Task-3^−/− cells during cyanide exposure (n= 6 patches).