Table 1. In vitro screening data against three different trypanosomatids.

ID No	T.b.rhod.a	SI	T. cruzib	SI	L.don. axen.c	SI	Cytotox. L6	Bnz/Comp.	Chemical Structure
	IC-50 μM		IC-50 μM		IC-50 μM		IC-50 μM
1	1.38	>14	0.34	>562	58.15	>3	>191.1	4.6
2	1.2	99	0.412	287	44.45	3	118.4	3.8
3	5.33	10	0.04	1320	25.92	2	52.79	39.2
4	49.6	5.2	1.678	152	>100	1	>100	0.9
5	35.65	4.3	0.848	180	>315	<1	152.68	1.9
6	1.34	114.6	8.8	17.5	>315	<1	153.9	0.2
7	0.231	180.2	0.048	868	11.35	3.7	41.7	32.7
8	23.85	5.1	0.419	289	173.45	<1	121.34	3.7
9	35.56	3.5	0.556	224.5	>238.66	<1	124.8	2.8
10	10.46	10.8	0.828	137	180.2	<1	113.3	2.5
11	0.78	42.9	0.286	117	23.9	1.4	33.5	7.1
12	15.62	13	4.7	43.1	>290	<1	202.9	0.3
13	62.4	2.2	34.4	4	186.4	<1	137.1	0.1
14	10.74	7.4	9.4	8.4	149.5	<1	79.2	0.2
15	0.204	500	0.059	1725	4.49	22.7	101.9	26.6
16	0.954	170	0.566	287	20	8.1	162.5	3.6
17	0.355	125.6	0.142	314	4.1	10.9	44.54	14.4
18	7.61	6.5	3.23	15.3	4.27	11.6	49.5	0.6
19	52.5	3.4	4.88	36.1	11.4	15.4	176	0.4
20	3.87	11.1	1.75	24.6	7.3	5.5	43	1.2

^aSTIB 900 trypomastigotes;

^bTulahuen C4 amastigotes;

^cMHOM-ET-67/L82 amastigotes; Cytotoxicity was measured in L6 cells. SI = IC₅₀ in L6 cells / IC₅₀ in each type of parasites. The reference compounds were: Melarsoprol (T. b. rhodesiense; IC₅₀ = 0.011 0.004), Benznidazole (Bnz; T. cruzi; IC₅₀ = 1.85 0.33) and Miltefosine (L. donovani; IC₅₀ = 0.378 0.011). Bnz/comp.: The ratio of IC₅₀ values of Benznidazole and each tested compound against T. cruzi. The IC₅₀ values are the means of two independent assays and varies less than ± 50%. Green color indicates active compounds, light red, moderately active, light blue, toxic and no color, inactive. Compounds 1-3 have been described before [10].