Table 1.
Parameter | Experiment 1 | Experiment 2—development | Experiment 2—settlement assay | Experiment 3 | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Control | pH 7.6 | Control | pH 7.9 | pH7.7 | Control | pH 7.9 | pH 7.7 | Control | pH 7.9 | pH 7.7 | pH 7.5 | |
pH (NBS) | 8.17 (±0.02) | 7.56 (±0.02) | 8.05 (±0.05) | 7.85 (±0.13) | 7.65 (±0.09) | 7.95 (±0.07) | 7.83 (±0.02) | 7.73 (±0.04) | 8.14 (±0.03) | 7.91 (±0.06) | 7.70 (±0.07) | 7.49 (±0.04) |
Total alkalinity (mM Kg−1) | 2,232 (±92) | 2,229 (±31) | 2,290 (±150) | 2,286 (±87) | 2,307 (±82) | 2,241 (±130) | 2,320 (±38) | 2,274 (±43) | 2,201 (±15) | 2,203 (±40) | 2,170 (±27) | 2,224 (±34) |
pCO2 (μatm) (calculated) | 425.3 (±25) | 2,035.8 (±124) | 604.7 (±111) | 1,015.9 (±317) | 1,691.0 (±419) | 769.7 (±167) | 1,085.1 (±80) | 1,367.0 (±153) | 455.3 (±39) | 838.9 (±134) | 1,404.6 (±240) | 2,407.0 (±256) |
Ωar (calculated) | 2.99 (±0.12) | 0.86 (±0.05) | 2.44 (±0.43) | 1.63 (±0.58) | 1.08 (±0.31) | 1.96 (±0.43) | 1.59 (±0.10) | 1.26 (±0.14) | 2.78 (±0.18) | 1.78 (±0.26) | 1.13 (±0.20) | 0.74 (±0.04) |
The partial pressure of carbon dioxide (pCO2) and aragonite saturation level (Ωar) was calculated from total alkalinity and pH using CO2SYS (Pierrot et al. 2006)