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. 2011 Jan 11;158(4):893–901. doi: 10.1007/s00227-010-1616-0

Table 1.

Characteristics of six microsatellite loci in a sample of 36 adult individuals of Shiner Perch

Locus Repeat motif Primer sequences Size range Number of alleles Ho He Exclusion probability
Cagg15 (AC)25

*F: Fam-TAGCACATTAGGATTCAAAA

R: ATTAGAGCAGGGTAGGATTA

 205–263 22 0.972 0.951 0.875
Cagg27 (GT)28

*F: Hex-GAAACATAATAAACAGCAGGAT

R: GAAGGAGATGAAAGGAACAA

 218–288 22 0.944 0.919 0.816
Cagg28 (AC)19

*F: Ned-CATACCGATGTTTAGACAGAA

R: ACAAATGGCAGTGAAAGGAG

 251–292 22 1.000 0.949 0.870
Cagg29 (AC)35

*F: Ned-ACAAGGACACCTGTCTCAAC

R: GAACTAACTCTTCCAGCAAA

 166–258 28 0.972 0.967 0.906
Cagg36 (GT)20

*F: Hex-GCAAGTTGGCATGTGATGAG

R: GCCTGGCAGATGTGAAAGAG

 104–142 15 0.944 0.910 0.794
Cagg50 (GT)36

*F: Fam-CGTGCAGACGGAAATGTGAT

R: ACCTGCTTCTTTCAGGGACA

 123–217 26 0.972 0.959 0.890

* The complete sequence of the primer includes M13 reverse tail (5′-GGAAACAGCTATGACCATG-3′) at its 5′—end. M13 reverse oligo labeled with Fam, Hex, or Ned was used in the PCR reaction