Table 1.
Estimation of AtDREB2A copy number in generations T0 and T1 using qPCR and the 2−ΔCt/2 method. The first column displays the selected events, the second and third columns display the results from the independent experiments, the fourth column lists the average of the two experiments, and the fifth, sixth and ninth columns show the standard deviation (SD), coefficient of variation (CV%) and estimated copy number, respectively.
| Sample | First experiment | Second experiment | Average 2−ΔCt | SD 2−ΔCt | CV% 2−ΔCt | SD+ | SD- | Nº of copies |
|---|---|---|---|---|---|---|---|---|
| P 1397 | 15.95 | 10.35 | 13.15 | 3.96 | 30.15 | 17.11 | 9.18 | 9 to 17 |
| P 7186 | 8.39 | 5.73 | 7.06 | 1.88 | 26.60 | 8.94 | 5.18 | 5 to 9 |
| P 7413 | 4.95 | 3.98 | 4.47 | 0.69 | 15.45 | 5.16 | 3.78 | 4 to 5 |
| P 2193 | 966.38 | 225.89 | 596.14 | 523.61 | 87.83 | 1119.74 | 72.53 | High** |
| P 7195 | 9.36 | 6.74 | 8.05 | 1.85 | 23.00 | 9.91 | 6.20 | 6 to 10 |
| P 7417 | 4.05 | 2.92 | 3.49 | 0.80 | 22.95 | 4.29 | 2.69 | 3 to 4 |
| P 7212 | 6.84 | 5.09 | 5.97 | 1.24 | 20.75 | 7.21 | 4.73 | 5 to 7 |
| P 7418 | 7.9 | 5.66 | 6.78 | 1.58 | 23.44 | 8.37 | 5.19 | 5 to 8 |
| P 7231 | 6.23 | 4.25 | 5.24 | 1.40 | 26.72 | 6.64 | 3.84 | 4 to 7 |
| P 7430 | 4.33 | 3.2 | 3.77 | 0.80 | 21.13 | 4.56 | 2.97 | 3 to 5 |
| P 7256 | 9.44 | 4.88 | 7.16 | 3.22 | 45.12 | 10.39 | 3.93 | 4 to 10 |
| P 7374 | 3 12 | 2.32 | 2.72 | 0.57 | 20.86 | 3.29 | 2.15 | 2 to 4 |
| P 7431 | 14 | 8.56 | 11.28 | 3.84 | 34.07 | 15.12 | 7.44 | 7 to 15 |
| P 7174 | 6.17 | 3.88 | 5.03 | 1.62 | 32.26 | 6.65 | 3.41 | 3 to 7 |
| P 7393 | 4.61 | 3.15 | 3.88 | 1.03 | 26.62 | 4.91 | 2.85 | 3 to 5 |
| P 7531 | 9.23 | 5.91 | 7.57 | 2.34 | 30.94 | 9.91 | 5.23 | 5 to 10 |
| P 1397.1 (T1)* | 0.07 | 0.05 | 0.06 | 0.01 | 20.41 | 0.07 | 0.05 | Negative*** |
| P 1397.2 (T1) | 2.94 | 1.7 | 2.32 | 0.88 | 37.85 | 3.20 | 1.44 | 1 to 3 |
| P 2193.1 (T1) | 0.76 | 0.53 | 0.65 | 0.16 | 25.29 | 0.81 | 0.48 | Negative |
| P 2193.2 (T1) | 4.14 | 2.12 | 3.13 | 1.43 | 45.68 | 4.56 | 1.70 | 2 to 5 |
Samples identified with T1 descended from the T0 generation (i.e., generation T1). The copy number in generation T1 was divided by two because these plants were hemizygous. The number followed by a dot in T1 plants indicates the two descendants analyzed of each planta (P 1397 and P 2193), here denominated dot 1 and dot 2.
High fluorescence values caused signal saturation that led to imprecise results; in this case, the plants were considered to have > 50 copies.
Samples with values < 1 were considered to be negative.